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When it comes to cell culture, the importance of choosing the right plates cannot be overstated. Not all plates are created equal, and making the wrong choice can lead to poor results, wasted time and resources, and even harm to your cell cultures. However, with the plethora of options available in the market, it can be difficult to know where to start. In this blog, we will guide you through the key factors to consider when choosing well cell culture plates.
Material.
The material of the plate can have a significant impact on the quality of your cell culture, so it's important to choose one that is compatible with your experiment. Common materials include polystyrene, polypropylene, and glass. Polystyrene plates are the most commonly used and are suitable for most cell types. Polypropylene plates are more durable and can withstand higher temperatures, making them suitable for heat-shock and other stress-inducing experiments. Glass plates are used for specialized applications, such as high-resolution microscopy, and offer a superior optical quality.
Coating.
The coating of the plate will also affect the attachment and growth of your cells. Common coatings include collagen, gelatin, and polylysine. Collagen-coated plates are suitable for cells such as fibroblasts and endothelial cells. Gelatin-coated plates are used for embryonic stem cells and other sensitive cell types. Polylysine-coated plates enhance attachment of cells that have difficulty adhering to uncoated plates. Some plates are also uncoated, which is suitable for cells that naturally attach to surfaces.
Surface area and well size.
The amount of surface area available for your cells to grow is important to consider to avoid overcrowding. Different industries will fit well with different-sized wells. Tissue culture plates generally come in 6-, 12- and 24-well formats, with larger well plates available for higher-throughput experiments. Organizations concerned primarily with drug discovery may prefer to use 384 or 1536-well plates, which can accommodate thousands of tiny wells for highly efficient screenings.
Sterility.
Sterility is another critical factor in cell culture plates. Plates must be sterilized before use and should have low endotoxin levels, which can negatively impacting the growth of your cells. It is highly imperative that you verify to ensure that the plates are sterilized, otherwise, they can cause to contaminate the cell cultures, which can waste lots of money and time. Sterile individually wrapped plates are the best options for reducing the risk of contamination and enhancing reproducibility.
Transparency.
The transparency of cell culture plates can impact the quality of imaging and microscopy. Clear plates are great for experiments that don't require microscopy examination. When frequent analyses are to be done, assay or clear-bottom plates will be suitable. For applications that require fluorescence or high-resolution microscopy, optically clear plates are the best fit. Black dishes are widely used for fluorescence assays because the dark background minimizes background fluorescence.
Durability.
Durability is an often-overlooked factor to consider in cell culture plates. The tendency for customers to lean towards the cheaper alternatives is common, but these choices can become fragile and crack easily upon handling, without necessarily saving and economies costs. Durability "can win" with time, effort, and cost-saving over the long term. Be sure to choose plates that are sturdy enough to withstand multiple rounds of experimentation, reagent exposure, and handling. Studying evaluations or evaluations enabling durability measures for several plates may offer insights on product durability.
Conclusion.
The choice of cell culture plates is an essential aspect to consider if you want to get the best results from your experiments. The material, coating, surface area, well size, transparency, sterility, and durability of the plates all contribute to the success of your cell culture. Ultimately, it's important to select plates that are appropriately suited for your specific experimental themes, whether it be drug discovery, tissue engineering, or diagnostic research. Make wise decisions with these factors in mind, and your cell culture is almost certain to flourish.
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